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PDB | 2LSV | The NMR high resolution structure of yeast Tah1 in complex with the Hsp90 C-terminal tail

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Internal ID
44245
PDB Entry ID
2LSV
Title
The NMR high resolution structure of yeast Tah1 in complex with the Hsp90 C-terminal tail
Authors
Back, R.,Dominguez, C.,Rothe, B.,Bobo, C.,Beaufils, C.,Morera, S.,Meyer, P.,Charpentier, B.,Branlant, C.,Allain, F.,Manival, X.
Primary Citation
AuthorsBack, R., Dominguez, C., Rothe, B., Bobo, C., Beaufils, C., Morera, S., Meyer, P., Charpentier, B., Branlant, C., Allain, F.H., Manival, X.
TitleHigh-Resolution Structural Analysis Shows How Tah1 Tethers Hsp90 to the R2TP Complex.
JournalStructure, v.21, pp.1834 - 1847, 2013
AbstractView
Pubmed24012479
DOI10.1016/j.str.2013.07.024
 
History
Deposition: 2012-05-07Release: 2013-05-22Last Modified: 2013-10-30
Experimental Method
TypeSOLUTION NMR
Unit Cell
Length [Å]abc
Angles [°]alphabetagamma
NMR Ensemble
Conformers Calculated200Conformers Submitted20Selection Criteriastructures with the least restraint violations
NMR Refine
Methodsimulated annealing
Molecular Description
PolymerMoleculeMutationFragmentChainsEC NumberOther details
1TPR repeat-containing protein associated with Hsp90A
2ATP-dependent molecular chaperone HSP82C-terminal tailB
Functional Class
Source
PolymerScientific NameCommon NameExpression System
1Saccharomyces cerevisiaeBaker's yeastEscherichia coli
Related PDB Entries
IDDetails
2LSU
SCOP Classification
CATH Classification
Structural Details
KeywordCHAPERONE-BINDING PROTEIN/CHAPERONE
TextCHAPERONE-BINDING PROTEIN-CHAPERONE complex
 
Polymeric Molecules
Chain A
DescriptionTPR repeat-containing protein associated with Hsp90
Polymer typepolypeptide
Formula Weight12393.1
Source Methodgenetically manipulated
Entity Name Sysn/a
 
Chain B
DescriptionATP-dependent molecular chaperone HSP82
FragmentC-terminal tail
Polymer typepolypeptide
Formula Weight1038.0
Source Methodsynthetic
Entity Name82 kDa heat shock protein, Heat shock protein Hsp90 heat-inducible isoform
Entity Name Sysn/a
 
Entity Poly
EntityChiralityLinkageMonomer# Mon.StrandTypeDetails
1n/anonon/aApolypeptide(L)n/a
2n/anonon/aBpolypeptide(L)n/a
Natural/Genetically Modified Source
Common NameBaker's yeast
Scientific NameSaccharomyces cerevisiae
Source Genusn/a
GeneTAH1, YCR060W, YCR60W
Host Common Namen/a
Host Scientific NameEscherichia coli
Host Genusn/a
Host Cell Linen/a
Host Strainn/a
Host Vector Typeplasmid
Host PlasmidpKHS
 
Unit Cell
Length a (Å)Length b Å)Length c (Å)
Angle Alpha (°)Angle Beta (°)Angle Gamma (°)
Spectrometer Info
Field StrengthManufacturerModel
500BrukerAVIII
700BrukerAVIII
900BrukerAvance
Sample Conditions
Sample ContentsSolvent SystemIonic StrengthpHPressurePressure UnitsTemperature
1 mM [U-100% 13C; U-100% 15N] protein_1, 1 mM protein_2, 10 mM sodium phosphate, 150 mM sodium chloride, 95% H2O/5% D2O95% H2O/5% D2O0.157.2ambientn/a288
1 mM [U-100% 13C; U-100% 15N] protein_1, 1 mM protein_2, 10 mM sodium phosphate, 150 mM sodium chloride, 100% D2O100% D2O0.157.2ambientn/a288
NMR Software
Author(s)ClassificationSoftware Name
Bruker BiospincollectionTOPSPIN
Bruker BiospinprocessingTOPSPIN
Goddardchemical shift assignmentSPARKY
Guntert, Mumenthaler and Wuthrichstructure solutionCYANA
Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and KollmanrefinementAMBER
NMR Ensemble Information
Conformers Calculated Total Number200
Conformers Submitted Total Number20
Conformer Selection Criteria structures with the least restraint violations
 
NMR Refinement Information
MethodDetails
simulated annealingn/a
NMR Exptl Information
Conditions IDExperiment IDSolution IDType
1112D 1H-15N HSQC
1213D HNCA
1312D 1H-13C HSQC
1413D H(CCO)NH
1512D F1-filtered, F2-filtered NOESY
1612D 1H-13C HSQC aliphatic
1713D 13C F1-filtered, F3 edited NOESY
1813D 15N F1-filtered, F2 edited NOESY
1922D 1H-13C HSQC aliphatic
11022D 1H-13C HSQC aromatic
11122D F1-filtered, F2-filtered NOESY
11222D 13C F1-filtered, F2-edited NOESY
11323D 13C F1-filtered, F3-edited NOESY aliphatic
11423D 13C F1-filtered, F3-edited NOESY aromatic
11512D 1H-15N HSQC
11613D 1H-13C NOESY aliphatic
11713D 1H-13C NOESY aromatic
11813D 1H-15N NOESY
11912D F1-filterd, F2-filtered TOCSY
12012D 1H-13C HSQC aromatic
Sequence Chain
A
Sequence
  1   SQFEKQKEQG  NSLFKQGLYR  EAVHCYDQLI  TAQPQNPVGY  SNKAMALIKL  GEYTQAIQMC

 61   QQGLRYTSTA  EHVAIRSKLQ  YRLELAQGAV  GSVQIPVVEV  DELPEGYDRS  

Sequence Chain
B
Sequence
1   ADTEMEEVD